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. 2004 Oct;70(10):6247–6256. doi: 10.1128/AEM.70.10.6247-6256.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 3. — Identification of I-CeuI and SfiI fragments from B. subtilis (natto) strains. (A) I-CeuI digests of genomic DNA of four B. subtilis (natto) strains. I-CeuI digests of the strains indicated were electrophoresed. Lanes Y and M contained yeast genome DNA and lambda phage DNA, respectively, as markers; sizes are indicated on the left and the right. I-CeuI fragments of the Marburg 168 genomic DNA designated in reference 44 are indicated by arrowheads. The fragment-like band in BEST195 indicated by an arrowhead was an artifact because it was not reproduced in separate running conditions. The running conditions for the gel on the left were 3 V/cm, a pulse time of 8 min, and a running time of 44 h. Resolution of the I-CeuI fragments smaller than 150 kb is shown in the gel on the right, for which the running conditions were as follows: 3 V/cm, a pulse time of 3 min, and a running time of 32 h. (B) SfiI digests of four B. subtilis (natto) strains. SfiI fragments of different strains were resolved by pulsed-field gel electrophoresis with the following running conditions: 3 V/cm, a pulse time of 24 s, and a running time of 56 h (left panel) and 3 V/cm, a pulse time of 6 s, and a running time of 24 h (right panel). Resolution of the SfiI restriction fragments smaller than 100 kb is shown in the right panel. Lanes M contained lambda phage DNA as a size marker. (C) Schematic diagram of the SfiI fragments of the four B. subtilis (natto) strains. All the SfiI fragments determined experimentally are included. Sizes are shown in Table 2.

FIG. 3. — Identification of I-CeuI and SfiI fragments from B. subtilis (natto) strains. (A) I-CeuI digests of genomic DNA of four B. subtilis (natto) strains. I-CeuI digests of the strains indicated were electrophoresed. Lanes Y and M contained yeast genome DNA and lambda phage DNA, respectively, as markers; sizes are indicated on the left and the right. I-CeuI fragments of the Marburg 168 genomic DNA designated in reference 44 are indicated by arrowheads. The fragment-like band in BEST195 indicated by an arrowhead was an artifact because it was not reproduced in separate running conditions. The running conditions for the gel on the left were 3 V/cm, a pulse time of 8 min, and a running time of 44 h. Resolution of the I-CeuI fragments smaller than 150 kb is shown in the gel on the right, for which the running conditions were as follows: 3 V/cm, a pulse time of 3 min, and a running time of 32 h. (B) SfiI digests of four B. subtilis (natto) strains. SfiI fragments of different strains were resolved by pulsed-field gel electrophoresis with the following running conditions: 3 V/cm, a pulse time of 24 s, and a running time of 56 h (left panel) and 3 V/cm, a pulse time of 6 s, and a running time of 24 h (right panel). Resolution of the SfiI restriction fragments smaller than 100 kb is shown in the right panel. Lanes M contained lambda phage DNA as a size marker. (C) Schematic diagram of the SfiI fragments of the four B. subtilis (natto) strains. All the SfiI fragments determined experimentally are included. Sizes are shown in Table 2.