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. 2016 Dec 20;7(1):35–43. doi: 10.1002/2211-5463.12147

Figure 2.

Figure 2

T0901317 sensitizes gefitinib by inducing apoptosis and cell cycle arrest in A549 cells. After treatment with gefitinib (5 μm) and/or T0901317 (5 μm) for 4 days, cell cycle and cell apoptosis were analyzed. (A) Cell apoptosis was analyzed by flow cytometer with annexin V/PI staining. *P < 0.001 versus untreated groups; # P < 0.001 between two groups. (B) Flow cytometry analysis of cell cycle distribution. (C) Colony formation assays were performed in A549 cells. *P < 0.01, **P < 0.001 versus untreated groups; # P < 0.01, ## P < 0.001 between two groups. (D) Western blot analyses were performed using the indicated antibodies.