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. 2016 Dec 5;7(1):64–73. doi: 10.1002/2211-5463.12156

Figure 6.

Figure 6

Phosphodiesterase inhibitors increase colocalisation of HSP20 and in SH‐SY5Y cells. (A) SH‐SY5Y cells were incubated with either 10 μm rolipram (Rol), 1 μm sildenafil (Sil), 1 μm PF‐04447943 (PF) or 1 μm BAY73‐6691 (BAY) for 15 min prior to methanol fixation and staining with antibodies against phospho‐HSP20 (pS16‐HSP20) and the C‐terminal domain of APP (APP‐CTD) which contains the Aβ epitope. Scale bar = 10 μm. (B) Pearson's Correlation coefficient (PCC) for the colocalisation of phosphor‐HSP20 and Aβ were calculated in cells treated with PDE inhibitors. Student's t‐test was used to evaluate changes compared to DMSO only controls. (*P < 0.05, Student t‐test, n = 3).