Figure 4.

Thin layer chromatography (TLC) analysis of alginate oligosaccharides in fermentation broth. (A) Samples from different fermentation time in three media with 1% inoculums. (B) Samples from different fermentation time in three media with 10% inoculums. (C) Samples from different fermentation time in seaweed water extract (SWE) medium with controlling pH at 7.0. Aliquot samples were taken at the interval of 4 hr and then spotted on the TLC plate. Std: alginate oligosaccharide standards. DP2‐DP7: the mannuronic acid sodium salt dimer, trimer, tetramer, and heptamer. Three media were ALG medium, ALG + SWE medium, and SWE medium, respectively. Rectangles showed the times with maximum concentration of alginate oligosaccharides in different media