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. 2016 Oct 20;500:62–70. doi: 10.1016/j.virol.2016.10.007

Fig. 2.

Fig. 2.

Replication of influenza A viruses in Calu-3 cells. A) Calu-3 cells were infected by the traditional liquid route (dashed line) or the aerosol route (solid line) at the target MOI with the viruses shown, and cultured at 37 °C or 33 °C. Culture supernatants were collected at the indicated times p.i., and titers were determined by standard plaque assay to quantify infectious virus. The limit of detection was 10 PFU. Error bars indicate standard deviation. Lines represent positive wells (infectious virus detected at two sequential timepoints or at 96 h alone, 3/3 unless otherwise noted) only. Cultures with 2/3 positive wells: Thai/16 33 °C aerosol 1×10−6, liquid 1×10−7; Anhui/1 33 °C aerosol 1×10−6, liquid 1×10−7; Panama/99 37 °C aerosol 1×10−6. Cultures with 1/3 positive wells: Thai/16 37 °C liquid 1×10−8; Panama/99 33 °C aerosol 1×10−5 and 1×10−6. B) Exact inoculum dose (PFU) and MOI for each infection shown in panel..