Fig. 3.
Effect of TNFα and AICAR on AMPK activity in 3T3-L1 adipocytes
3T3-L1 cells were incubated with 50 ng/ml of TNFα (T), a combination of 2 mM of AICAR with 50 ng/ml of TNFα (TA), or 2 mM of AICAR alone (A). After 16 h, AMPK activity was assayed. The activity was expressed as percent of the controls ± SD (n = 5). *: p < 0.005. **: p < 0.001.