Figure 3.

S1PR1 is required for persistent HLMVEC of migration in response to fluid flow. (a) Box-and-whisker plots showing the radial persistence of tracked cells that were untreated (−) or treated with 1 µM W146 (+) in the absence of fluid flow for 20 hours. Average cell displacements were 200±60 and 300±100 μm (mean±standard deviation) for untreated and W146-treated cells, respectively. N = 50 cells. (b) Schematic detailing the calculation of radial persistence for migrating HLMVECs. Analogous to figure 1d, the radial migration distance (d_r, green) is calculated with respect to the jet centre, while the total distance travelled d_t is indicated by a dashed line. The radial persistence d_r/d_t ranges from −1 to 1. (c) Box-and-whisker plots showing the radial persistence of tracked cells that were untreated (−) or treated with 1 µM W146 (+), binned by initial position in response to 20 h of impinging flow at a maximum WSS of 9 dynes cm⁻². N = 200 cells per condition, taken from two independent experiments. (d) Box-and-whisker plots showing the radial persistence of tracked cells that were treated with scrambled siRNA (−) or treated with siRNA targeting S1PR1 (+), binned by initial starting position in response to 20 h of impinging flow at a maximum WSS of 9 dynes cm⁻². N = 200 cells per condition, taken from two independent experiments.