Table 3.
Expression of miRNA and their experimentally validated mRNA targets
| miRNA | Altered in AR* (QPCR) |
Experimentally verified target (miRTarBase) |
Altered in AR* (Microarrays) |
miRNA-mRNA correlation (Pearson, p<0.05) |
Normal kidney protein expression (IHC, Human Protein Atlas) |
Cell specific expression (BioGPS, 3× median, microarrays) |
|
|---|---|---|---|---|---|---|---|
| Glomerulus | Tubule | ||||||
| miR25 | Up | PRMT5 | Down | − | + | +++ | 721 Bcell, CD34+ cell, Bronchial epithelial cell |
| TP53 | Down | − | − | − | 721 Bcell | ||
| miR181a | Down | CDX2 | Up | − | − | − | No cell specificity |
| ATM | Up | − | +++ | +++ | CD8+ Tcell, CD4+ Tcell, CD56+ NK cell | ||
| HIPK2 | Up | − | ++ | +++ | No cell specificity | ||
| miR204 | Down | TGFBR1 | Up | −0.51 | + | +++ | No cell specificity |
| TGFBR2 | Up | −0.44 | No data | No data | Adipocyte, smooth muscle cell, CD4+ Tcell | ||
| SNAI1 | Up | −0.55 | +++ | +++ | No cell specificity | ||
| SPDEF | Up | − | No data | No data | No cell specificity | ||
| miR192 | Down | MAD2L1 | Up | − | − | +++ | 721 Bcell, CD105+ Endothelial cell, small intestine |
| HRH1 | Up | −0.53 | + | ++ | No cell specificity | ||
| LMNB2 | Up | −0.58 | No data | No data | 721 Bcell, CD34+ cell, CD105+ endothelial cell | ||
| DTL | Up | − | − | −/+ | CD105+ Endothelial cell, 721 Bcell, CD34+ cell | ||
| miR10b* | Down | NCOR2 | Up | − | + | +++ | No cell specificity |
| miR142-3p | Up | RAC1 | Down | − | −/+ | + | Amygdala |
| miR215 | Down | ACVR2B | Up | − | + | ++ | Placenta |
| miR342-3p | Up | No targets | − | − | − | − | − |
| miR615-3p | Down | No targets | − | − | − | − | − |
Note: only significantly altered miRNA, mRNA targets and correlations listed. Official gene symbol listed for mRNA targets.
*
= significantly altered compared to normal. IHC = immunohistochemistry,
−
= negative/ absent,
−/+
= very weak,
+
= weak,
++
=moderate,
+++
=strong