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. 2016 Nov 7;27(22):3395–3404. doi: 10.1091/mbc.E16-02-0130

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© 2016 Wynne and Funabiki. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Centromere and core kinetochore configurations are both sensitive to microtubule attachment status. (A) hTERT-RPE1 cells in prometaphase and metaphase. Locations of higher- magnification images of sister kinetochore pairs (scale bars, 0.2 μm) indicated by boxes. (B) Quantification of kinetochore shapes in human cells and Xenopus (C–E) hTERT-RPE1 cells arrested in mitosis for 4 h in the presence or absence of nocodazole and ZM447439 (scale bars, 1 μm). Locations of higher-magnification images of sister kinetochore pairs (scale bars, 0.2 μm) indicated by boxes. Quantification of CENP-C foci lengths from nuclei in C is shown in D.