FIGURE 7:

Exchangeable GTP content of microtubules probed with ³²P-GTP and with an EB1 protein. (A) Image shows ³²P nucleotide content of wild-type (grown with GTP or dGTP) or β:C12A mutant microtubules (grown with GTP) analyzed by TLC. Data from four independent trials. Wild-type and β:C12A microtubules growing with GTP show comparable ³²P-GTP content; wild-type microtubules growing with dGTP show reduced ³²P-GTP content. (B) Representative images of microtubules growing in the presence of Bim1-GFP. Scale bar, 1 μm. (C) Plots of average Bim1-GFP intensity (n = 10) on microtubules. Wild-type and β:C12A microtubules growing with GTP show very similar Bim1 comets, whereas wild-type microtubules growing with dGTP show a narrower comet.