Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 7;27(22):3601–3615. doi: 10.1091/mbc.E16-06-0404

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Tsekouras et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — Synthetic photobleaching time trace for 50 fluorophores, with μ_f /σ_f = 10 for the single fluorophore, illustrating how noise obscures steps when many fluorophores are active at the start of the trace. Although it is easy to resolve individual steps late in the trace when fluorophore numbers are low, for >10 or so fluorophores, the steps are strongly obscured by additive noise. Here μ_f = 2.0, σ_f = 0.2, μ_b = 20.0, and σ_b = 0 (quantities defined in the text). Both time and fluorescence intensity are in arbitrary units.