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. 2016 Nov 7;27(22):3601–3615. doi: 10.1091/mbc.E16-06-0404

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© 2016 Tsekouras et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 10: — Our analysis of a trace obtained from HeLa cells transfected with Alexa Fluor 647–tagged microRNA-7. Our method locates seven steps in this data set, including two steps ascribed to blinking-on/off events. By contrast, the original analysis method found only three steps. The difference may be due to the fact that the original analysis method used filtering and averaging, which smooths out features of short duration, such as blinking.