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. 2016 Nov 7;27(22):3601–3615. doi: 10.1091/mbc.E16-06-0404

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© 2016 Tsekouras et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 12: — Long time trace also obtained from HeLa cells transfected with Alexa Fluor 647–tagged microRNA-7. Our method marks ∼30 steps in this data set. The original analysis method found this data set intractable even after filtering. Our method finds not only many steps but also multiple blinking and overlapping events. Photobleaching events that are very close in time register as overlapping events if the data acquisition rate is longer than the interval between successive photobleachings.