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. 2016 Nov 7;27(22):3601–3615. doi: 10.1091/mbc.E16-06-0404

FIGURE 9:

FIGURE 9:

Our analysis of a trace obtained on Alexa Fluor 555–tagged RAD51 proteins. In keeping with our expectation for how noise should change as more fluorophores are active, we identify two steps and determine that the small rise at the very start of the trace arises from noise, contrary to the analysis in van Mameren et al. (2009). At low noise, our findings are again consistent with steps identifiable by eye.