TABLE 1:
Output parameters from DoC, DBSCAN, and Clus-DoC.
| Algorithm | Output parameter |
|---|---|
| DoC | DoC score for each localization for protein A and protein B |
| Percentage of colocalized points for protein A and protein B (threshold based) | |
| DBSCAN plus contour mapping | Percentage of localizations in clusters |
| Number of clusters in ROI | |
| Number of localizations per cluster | |
| Circularity of clusters | |
| Size of clusters | |
| Absolute molecular density within clusters | |
| Relative molecular density within clusters | |
| Clus-DoC | Distinguishes colocalized versus noncolocalized clusters for protein A or protein B: |
| - Number of molecules per cluster | |
| - Circularity of clusters | |
| - Size of clusters | |
| - Absolute or relative density within clusters | |
| - Distribution of total or colocalized molecules between colocalized and noncolocalized clusters | |
| Distinguishes cluster populations based on density/size/number of localizations per cluster: | |
| - Average DoC score | |
| - Distribution of DoC scores | |
| - Percentage of colocalized molecules within each cluster population | |
| - Distribution of colocalized molecules between the subpopulations | |
| - Cluster morphology parameters | |
| Isolates nonclustered molecules: | |
| - Average DoC score | |
| - Proportion of total molecules and colocalized molecules that are not in clusters | |
| - Percentage of nonclustered molecules that are colocalized |