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. 2016 Nov 7;27(22):3637–3644. doi: 10.1091/mbc.E16-07-0525

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© 2016 Hummer et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Extraction of photophysical parameters from blinking statistics of (A) mEos2 monomers and (B, C) CTLA-4 dimers. The measured counts (Fricke et al., 2015a) c(n) are shown as boxes. (A) For the mEos2 monomer, the maximum-likelihood fits for simple counting statistics (Eq. 11; thick green line; p = p₀ = 0.289) and for the more complex two-parameter statistics (Eq. 7; thin blue line; p = 0.286 and p₀ = 0.295) are nearly indistinguishable. (B) For the CTLA-4 dimer, we obtain a value of q ≈ 0.295 with p = p₀ = 0.289 fixed. Insets, cumulative distribution functions (experiment: symbols; theory: lines). (C) Isocontour lines of the log-likelihood in simultaneous fits of p and q to CTLA-4 count statistics (solid/thin lines: contours −1/−2 relative to maximum). Δ indicates the difference of the respective maximum of the log-likelihood relative to the dimer model.