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. 2016 Nov 7;27(22):3645–3658. doi: 10.1091/mbc.E16-06-0425

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© 2016 Shelby et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Lipid anchorage of mEos3.2 constructs determines antigen-induced recruitment to IgE/FcεRI and the effects of cytoskeletal perturbation. (A) Representative two-color images of cells expressing PM-mEos3.2 (top) or mEos3.2-GG (bottom) sensitized with Dy654 IgE and stimulated for 6 min. (B) Average PM/IgE-FcεRI and GG/IgE-FcεRI cross-correlation fit parameters A and ξ as a function of stimulation time in RBL-2H3 cells. Cells were stimulated for 1, 3, 6, or 12 min with and without treatment with 1 μM latrunculin. Fit parameters are averaged over multiple cells for each time point (seven cells per time point for cells expressing both constructs and for both treatment conditions). Error bars denote SEM.