Figure 2.

Cocaine decreased microglial miR-124 levels in rPM. rPM were seeded into 6-well plates and exposed to various doses of cocaine (1–100 µM) for 24 h or with 10 µM of cocaine for the indicated time periods. After various treatments, cells were collected for RNA extraction and subjected to assessment of miR-124 by qPCR. (A and B) cocaine significantly decreased miR-124 levels in rPM cells. (C and D) Cocaine also increased DNMT1 levels in rPM. All experiments were done 4 times, independently. For all Western blots, β-actin served as protein loading control. Data are expressed as mean ± SEM and were analyzed using ANOVA (* P < 0.05 vs. control).