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. 2016 Oct 27;11(11):819–830. doi: 10.1080/15592294.2016.1232233

Figure 4.

Figure 4.

DNMT1 inhibitor 5-AZA reversed cocaine-mediated effects on DNMTs and miR-124. (A) BV-2 cells were seeded into 6-well plates and pre-treated with DNMTs inhibitor 5-AZA followed by cocaine exposure for another 24 h. DNMT1 mRNA and protein levels were determined by qPCR and Western blot, respectively. (A) DNMTs inhibitor 5-AZA significantly blocked cocaine-mediated upregulation on DNMT1 protein levels. (B, C, and D) DNMTs inhibitor 5-AZA significantly blocked cocaine-mediated upregulation on DNMT1, 3a, and 3b mRNA levels. (E) DNMTs inhibitor 5-AZA significantly blocked cocaine-mediated miR-124 downregulation in BV-2 cells. All experiments were done 4 times, independently. For all western blots, β-actin served as protein loading control. Data are expressed as mean ± SEM and were analyzed using ANOVA (* P < 0.05 vs. control; # P < 0.05 vs. cocaine-treated samples).