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. 2016 Oct 27;11(11):819–830. doi: 10.1080/15592294.2016.1232233

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Figure 6. — Cocaine increased methylation levels in pri-miR-124-2 promoter regions in BV-2 and adult isolated mouse microglia. BV-2 cells were exposed to cocaine (10 µM) for 24 h. Adult mouse microglia were purified from mice on a 7-day cocaine administration regimen. BV-2 cells and isolated adult mouse microglia were subjected to genomic DNA extraction. Genomic DNA was then treated with bisulfite for nucleotide conversion followed by bisulfite genomic DNA PCR and DNA sequencing. (A) Schematic of CpG islands located in the pri-miR-124-2 promoter region. (B and C) Cocaine significantly increased pri-miR-124-2 promoter methylation rates in BV-2 cells as well as in adult mouse microglia.