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. Author manuscript; available in PMC: 2017 Jan 9.
Published in final edited form as: Cell Rep. 2017 Jan 3;18(1):237–247. doi: 10.1016/j.celrep.2016.12.013

Figure 3. Recombinant scFv with VH AA sequences identified by MS from PV3a serum bind Dsg3.

Figure 3

(A) Two scFv (GB4P4-7 and GB8P3-8) with different VH AA sequences as identified in MS, synthesized and isolated as in Experimental Procedures, bind Dsg3 by ELISA. (B) Same two scFv bind cell surface of keratinocytes in monkey esophagus where Dsg3 locates. Scale bar, 10 μm (C) PV3a serum but not normal human serum inhibits binding of these scFv to Dsg3 on ELISA. See also Table S4.