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. 2017 Jan 1;28(1):120–127. doi: 10.1091/mbc.E16-09-0675

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© 2017 Jao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Gle1 is localized to the centrosome and basal body. (A) eGFP-tagged human Gle1 and Cetn4-TagRFP were transiently expressed from the injected RNA in the cells of dome-stage zebrafish embryos. eGFP-Gle1 localized to the NE (arrow) and the centrosome (Ce; arrowhead). (B) Confocal images of serum-starved human RPE-1 cells stained with antibodies against Gle1 and acetylated tubulin (AcTub; top) or Gle1 and PCNT (middle and bottom). Endogenous Gle1 localized to the ciliary base (top; arrowhead) and the centrosome (middle and bottom; arrowhead) as well as to NE (arrow). Scale bars: 10 μm (main images), 1 μm (insets).