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. 2017 Jan 1;28(1):120–127. doi: 10.1091/mbc.E16-09-0675

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© 2017 Jao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Gle1 is enriched around the mother centriole and intercalated with PCNT. (A, B) Confocal images of CETN-GFP RPE-1 cells stained with antibodies against Gle1 (A) or PCNT (B) show that Gle1 and PCNT are predominantly localized around the mother centriole at different cell cycle stages. (C) 3D-SIM images of interphase human RPE-1 cells stained with antibodies to Gle1 and PCNT (top) or Gle1 and CETN (bottom). The PCNT antibody recognizes the N-terminal portion of PCNT. Scale bars, 1 μm (A, B, all but bottom), 5 μm (A,B, bottom), 0.5 μm (C).