Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 1;28(1):152–160. doi: 10.1091/mbc.E16-09-0640

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Dar and Pucadyil. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 4: — Effects with an alternate dynamin construct lacking the PHD on native membranes. (A) The ΔPH-6xK construct has the entire PH domain deleted and replaced with a 6xLys linker. (B) Fluorescence micrographs showing WT and ΔPH-6xK on a p-Texas Red DHPE–labeled membrane tube composed of BPLE. White arrowheads mark sites of tube constriction. Scale bars, 5 μm. (C) Kymographs of scaffold assembly–induced tube constriction for WT and ΔPH-6xK on BPLE. Data are acquired in the tube fluorescence channel, and the decay in fluorescence intensity reflects assembly-induced constriction of the tube underlying the scaffold. (D) Single-pixel tube fluorescence changes in response to GTP addition to WT (blue) and ΔPH-6xK (red) scaffolds on BPLE. Fission time represents time between onset of constriction and the cut in the tube. (E) Fission time for WT and ΔPH-6xK. Data represent mean ± SD (n ≥15, N = 1, Student’s t test).