FIGURE 1:

Approach for labeling intracellular proteins with QDs. (A) Schema for labeling intracellular proteins with QDs. (B) Binding of HaloTag ligand–QD with HaloTag proteins in vitro. HaloTag ligand–QDs were incubated with the lysate of KRX cells expressing HaloTag–14-3-3β/α proteins and separated on an agarose gel. Samples on the right half of the gel were denatured by boiling the lysates before mixing with HaloTag ligand–QDs. “Original” refers to QDs with no conjugation reaction. (C) Snapshots of QD fluorescence in 3T3-L1 fibroblasts. Cells were immersed with QD-containing solution, and electroporation was performed with the indicated poration pulse voltages. Solid and dashed lines represent plasma and nuclear membranes, respectively. The Laplacian of Gaussian-filtered images. (D) Dependence of internalization of QD in 3T3-L1 fibroblasts on poration pulse voltage.