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. 2017 Jan 1;28(1):173–181. doi: 10.1091/mbc.E16-06-0413

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© 2017 Hatakeyama et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Effect of insulin on HaloTag–myosin movement. (A) Lifeact-Venus fluorescence before (left) and after 4 min of insulin stimulation (right) in 3T3-L1 fibroblasts. Membrane ruffling (arrows) and actin comet tails (arrowhead) were observed. Images are maximum intensity projections over five successive frames. (B) Magnified images of boxed regions shown in A. (C) MSD curves derived from the whole part of the trajectories having diffusion coefficients >0.027 μm²/s in cells expressing HaloTag-myosin Ic (left), HaloTag-myh9 (center), and HaloTag-myosin Vb (right) before (black) and 5 min after (red) insulin stimulation. Data are presented as mean ± SEM.