FIGURE 5:

p150 regulates Ki-67 localization throughout the cell cycle: a summary of dependence relationships. (A) HeLa S3 cells were stained with DAPI to visualize DNA (blue) and antibodies against Ki-67 (green) to demonstrate Ki-67 staining patterns throughout the cell cycle. (B) Hierarchy of known proteins controlling localization to the perichromosomal layer during mitosis. Proteins dependent on Ki-67 include pescadillo (PES-1; Sobecki et al., 2016), as well as NCL, NIFK, and the cPERPs (Booth et al., 2014). (C) p150 is required for Ki-67 localization to punctate foci during early G1 (Figure 3). (D) p150N is required for localization of the Ki-67 protein and NAD loci to nucleoli during interphase (Smith et al., 2014).