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. 2016 Sep 9;11(11):791–803. doi: 10.1080/15592294.2016.1230576

Figure 1.

Figure 1.

5-AZA treatment restores Cdkn1c expression in unresponsive cells. (A) qPCR analysis of the MeDIP assay performed on the KvDMR1 sub-region bound by MyoD (KvDMR1-MyoD) and on the Cdkn1c promoter in untreated (Ctr) and 5-AZA-treated samples. Values are expressed as percentages of Input DNA. Error bars indicate standard error of the mean (SEM) for each sample analyzed in triplicate. (B) RT-qPCR in unresponsive cells (C3H10T1/2 fibroblasts) infected with either the empty vector retrovirus (Vector) or the MyoD-encoding retrovirus (MyoD) and treated as above. Statistical significance: P < 0.01 (**). (C) RT-qPCR analysis of myogenin and MyoD expression in unresponsive cells infected with MyoD and treated as above. Values, relative to those of 18S RNA, are the mean of 4 independent experiments ± SEM. Statistical significance: ns (not significant). (D) Allele-specific RT-qPCR analysis of Cdkn1c expression in hybrid fibroblasts (C57BL/6 female x SD7 male) infected with MyoD and treated as above; values, normalized on 18S expression, are the mean of 3 independent experiments ± SEM. Statistical significance: ns. M: maternal; P: paternal.