Cdkn1c silencing in human cancer cells correlates with H3K9me2 enrichment at KvDMR1. (A) ChIP-qPCR analysis of H3K9me2 enrichment at KvDMR1 in MCF-7 and MDA-MB-231 (MDA). Values obtained for the enrichment were normalized to those of APOC-III promoter, used as a positive control, and expressed as percentages of Input chromatin. The results, derived from 2 independent experiments, are reported as mean ± SEM. Statistical significance: P < 0.05 (*). (B) RT-qPCR analysis of Cdkn1c expression in MCF-7 cells untreated (Ctr) or 5-AZA treated samples. Values, relative to those of GAPDH RNA, are the mean of 3 independent experiments ± SEM. Statistical significance: P < 0.05 (*).