Skip to main content
. Author manuscript; available in PMC: 2017 Jan 9.
Published in final edited form as: Methods Enzymol. 2010;476:329–349. doi: 10.1016/S0076-6879(10)76019-5

Figure 19.3.

Figure 19.3

Time-lapse movie of the developing Yolk sac vessels (cultures starting at E8.5). Yolk sac vessels of intact Flk1-myr::mCherrytg/tg (to visualize endothelial cell membrane); Flk1-H2B::eYFPtg/tg (to visualize endothelial cell nuclei) embryos are imaged on the LSM 5 LIVE confocal with a 25× objective (NA of 0.45). Images are taken every 6 min at three Z-planes (~30 μM) each with the 488 nm (0.3% power) and 561 nm (2.0% power) lasers to image eYFP and mCherry, respectively. Endothelial cell apoptosis (panels A-A″) and mitosis (panels B-B″) can be captured in movies by observing fragmentation of nuclei and division of a single nucleus into two nuclei, respectively. Beginning of chromatin condensation is observed in A′ (6 minutes after A), and nuclear fragmentation is observed in A″ (1 hour after A). Beginning of mitosis is observed in B′ (12 minutes after B) and formation of two separate nuclei is apparent in B″ (18 minutes after B).