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. 2017 Jan 9;12(1):e0169155. doi: 10.1371/journal.pone.0169155

Fig 2. mTORC1 is inhibited by rapamycin and shRNA against Raptor.

Fig 2

(A) Keratinocytes were incubated under normoxic and hypoxic conditions in the presence or absence of rapamycin (100 mM, SB) or the shRNA against Raptor (shRaptor). Western blotting was performed to analyze the expression of phosphorylated p70S6K (Thr389), p70S6K, phosphorylated 4E-BP1 (Thr70), 4E-BP1, and β-actin (loading control). (B) The graphs represent the mean ± SD (n = 3) of the relative integrated signals. N, normoxia. *P< 0.05 versus the N group. #P< 0.05 versus the hypoxia 6 h + DMSO group. (C) The graphs represent the mean ± SD (n = 3) of the relative integrated signals. N, normoxia. *P< 0.05 versus the N group. #P< 0.05 versus the hypoxia 6 h + shNC group.