FIG. 5.

Recovery of swimming ability of the TS PomA mutants. The fractions of swimming NMB190 cells expressing AAQQQ+L131F PomA (circles) and wild-type PomA (squares) are shown. The cell suspension was prepared in a manner similar to that for the measurement of swimming speed, except that it contained 25 μg of chloramphenicol/ml in TMN500 buffer. This cell suspension was incubated at 20°C for 10 min, then placed at 42°C, and returned to 20°C. The number of swimming cells was counted at the incubation temperature on a microwarm plate. The value was the average of results from two independent experiments.