Fig 3. Detection of different Cms strains in terms of their EPS levels by DAS-ELISA (Loewe kit).

Bacterial cells of three different strains (527 –Rough, 758 –Intermediate, NCPPB 4053 –Fluidal) were suspended: (1) in water, (2) washed three times with ddH₂O, (3) washed twice at pH = 2.5 and three times with ddH₂O, (4) washed twice at pH = 10.5 and three times with ddH₂O, (5) washed twice at pH = 2.5, twice at pH = 10.5, and three times with ddH₂O.Absorbance equal to at least two-fold the value of the blank sample was accepted as a positive result. All assays were performed twice in three replicates.