Table 2. Properties of the newly developed antibodies against Cms bacterial cells (strains NCPPB 4053, 758, 527) using mucoid and nonmucoid cells.

Titers required for detection were estimated in each of the cases by the PTA-ELISA method using 10⁵ CFU/ml of aqueous suspensions of bacteria and suspensions for which EPS components were gradually washed off.

IgG	Cms cell suspension treatment	IgG dilution							Blank(no IgG)
		1:500	1:2,000	1:8,000	1:32,000	1:128,000	1:512,000	1:2,048,000
IgG against mucoid Cms cells	Suspension in water	+	+	+	+	+	-	-	-
	Washing three times with sterile water	+	+	+	+	-	-	-	-
	Washing at pH 2.5	+	+	-	-	-	-	-	-
	Washing at pH 10.5	+	+	+	-	-	-	-	-
	Washing at pH 2.5 and 10.5	+	+	-	-	-	-	-	-
IgG against nonmucoid Cms cells	Suspension in water	+	+	+	+	-	-	-	-
	Washing three times with sterile water.	+	+	+	+	-	-	-	-
	Washing at pH 2.5	+	+	+	+	+	-	-	-
	Washing at pH 10.5	+	+	+	+	-	-	-	-
	Washing at pH 2.5 and 10.5	+	+	+	+	+	-	-	-

+ = positive result from PTA- (at least two-fold higher compared to negative control)

− = negative result from PTA-ELISA (less than two-fold higher compared to negative control)