Table 3. Comparison of the sensitivity of different ELISA tests for detecting Cms bacterial cells.

Strains tested were 527 and NCPPB 4053.

Bacterial suspension (CFU/ml)	Loewea		Agdiab		Adgenc		Newly developed IgG
							Against mucoid Cms cells		Against nonmucoid Cms cells
	527	NCPPB 4053	527	NCPPB 4053	527	NCPPB 4053	527	NCPPB 4053	527	NCPPB 4053
107	+	+	+	+	-	+	+	+	+	+
106	+	+	-	+	-	+	(+)	+	+	+
105	-	+	-	+	-	+	-	+	+	+
104	-	+	-	(+)	-	-	-	+	+	(+)
103	-	(+)	-	-	-	-	-	-	-	-
102	-	-	-	-	-	-	-	-	-	-
NC1	-	-	-	-	-	-	-	-	-	-
NC2	-	-	-	-	-	-	-	-	-	-

+ = positive result from ELISA (more than two-fold higher than the negative control)

(+) = weak positive (approximately two-fold higher than the negative control)

- = negative result from ELISA (less than two-fold higher than the negative control)

NC1—Negative control 1—Coating buffer

NC2—Negative control 2—Potato extract diluted 1:100 in coating buffer

^aLoewe kit containing polyclonal goat primary IgG against the Cms strain NCPPB 2140 and polyclonal goat secondary IgG conjugated with AP enzyme, DAS-ELISA method

^bAgdia kit containing monoclonal mouse primary IgG and polyclonal rabbit secondary IgG conjugated with alkaline phosphatase AP enzyme, DAS-ELISA method

^cAdgen kit containing polyclonal primary IgG-anti Cms and polyclonal secondary IgG conjugated with AP enzyme, PTA-ELISA method