Table 4. Detection of Clavibacter michiganensis subsp. sepedonicus with newly developed IgG directed against cells from this bacterial species without the exopolysaccharide layer (IgG-N-EPS) in the field.

	Rough Cms strains		Intermediate Cms strains		FluidalCms strains		Negative control
	527	LMG 6385	758	NCPPB 3158	NCPPB 2913	NCPPB 3326
Pathogenitya	**	*	*	*	**	***	-
Absorbance (405 nm)	0.360	0.158	0.250	0.182	0.280	0.350	0.045
SD	±0.009	±0.009	±0.005	±0.007	±0.01	±0.008	± 0.004
PCRb	+	+	+	+	+	+	-

Least significant difference (LSD) = 0.018 was determined using univariate analysis of variance at a significance level of 0.0001

Negative control—Non-inoculated

^aPathogenity of Cms strains determined by bioassay according to Directive 2006/56/EC [2].

^bPCR performed with PSA-1, PSA-R primers according to Pastrik [16], which is the only PCR procedure included in the Commission Directive 2006/56/EC on the control of potato ring rot [2].

*weakly pathogenic,

** pathogenic,

*** strongly pathogenic

+ = positive result from ELISA (at least two-fold higher compared to negative control)

- = negative result from ELISA (less than two-fold higher compared to negative control)