FIG. 7.

Requirement of Hfq for MicC repression of ompC. (A) Hfq binding to MicC RNA. Cell extracts were prepared from wild-type (BW25113) or hfq-1 mutant (GSO107) cells grown to an OD₆₀₀ of 0.6 in LB medium at 24°C. Immunoprecipitations were carried out with the wild-type extracts and an Hfq antiserum or a preimmune serum and were compared to total RNAs from 1/10 extract equivalents of the wild-type and hfq-1 mutant RNAs. The levels of MicC and the DsrA positive control were determined by Northern hybridization. (B) Levels of OmpC, OmpF, and OmpA proteins in wild-type and hfq-1 mutant strains without and with pAlter-Ex2 or pAE-micC grown to an OD₆₀₀ of 0.6 in LB medium at 24°C.