TABLE 3.
Genes showing prolonged induction upon cold shock
| Gene name and role | Gene product and/or function | Wild-type 15°C/37°C ratio at 1 h | Wild-type 15°C/37°C ratio at 5 h | Mutant/ wild-type ratio (15°C, 1 h) |
|---|---|---|---|---|
| Genes involved in membrane synthesis/function | ||||
| cheW | Chemotaxis protein CheW, adapter protein | 3.5 ± 0.05 | 5.4 ± 0.01 | |
| cheY | Chemotaxis protein CheY | 4.3 ± 0.09 | 3.6 ± 0.51 | |
| dcuA | Anaerobic C4-dicarboxylate transporter DcuA | 7.4 ± 0.36 | 7.3 ± 0.7 | |
| dmsC | Dimethylsulfoxide reductase chain C | 4.3 ± 0.09 | 7 ± 1 | |
| flg and fli | Flagellar proteins | 2.0-12 ± 0.5 | 4.0-13 ± 1 | 0.01-0.1 ± 0 |
| frdA | Fumarate reductase flavoprotein subunit | 20 ± 0.5 | 15 ± 1 | 0.2 ± 0.02 |
| frdB | Fumarate reductase iron-sulfur protein | 17 ± 0.9 | 11 ± 0.9 | |
| frdD | Fumarate reductase, 13-kDa membrane anchor protein | 6 ± 0.55 | 3.5 ± 0.7 | |
| glpQ | Glycerophosphodiester phosphodiesterase | 4.3 ± 0.13 | 5.5 ± 1 | |
| glpT | Glycerol-3-phosphate transport protein | 7.8 ± 0.33 | 6.1 ± 0.36 | |
| hybA | HybA protein | 8.4 ± 0.92 | 8 ± 1 | |
| hybB | HybB protein | 4.6 ± 0.23 | 11 ± 1.5 | |
| hybC | HybC protein | 4.5 ± 0.13 | 6 ± 0.6 | |
| hypB | Hydrogenase isoenzymes formation protein | 17.8 ± 1.37 | 17 ± 1.5 | |
| hypE | HypE protein | 5.3 ± 0.12 | 13 ± 1.3 | |
| nupG | Nucleoside-transporting protein NupG | 18.6 ± 2.28 | 15 ± 1.4 | |
| ompC | Outer membrane protein C precursor | 5 ± 0.1 | 4 ± 0.08 | 0.24 ± 0.02 |
| ompT | Proteinase VII precursor | 19.7 ± 1.25 | 57 ± 6 | |
| tap | Methyl-accepting chemotaxis protein II | 4.2 ± 0.12 | 4.8 ± 0.4 | |
| tar | Methyl-accepting chemotaxis protein II | 11.1 ± 0.85 | 7.8 ± 1 | |
| Genes involved in cell metabolism | ||||
| ackA | Acetate kinase | 4.2 ± 0 | 5.5 ± 0.02 | |
| ansB | Asparaginase | 10.8 ± 0.16 | 25.3 ± 3 | |
| asnB | Asparagine synthase | 5.1 ± 0.34 | 6 ± 2 | |
| cydA | Cytochrome d ubiquinol oxidase subunit I | 3.8 ± 0.35 | 5.7 ± 0.9 | |
| ftn | Ferritin | 13 ± 1 | 13 ± 0.9 | |
| fumB | Fumarate hydratase | 9.6 ± 1.34 | 9.6 ± 1.5 | |
| glpB | Glycerol-3-phosphate dehydrogenase | 20.3 ± 1.43 | 14.8 ± 0.28 | |
| glpC | Glycerol-3-phosphate dehydrogenase | 27.4 ± 3.7 | 14.1 ± 2.5 | |
| pflB | Formate C-acetyltransferase | 5.6 ± 0.46 | 5.5 ± 1.5 | |
| wrbA | Trp repressor binding protein | 15 ± 2 | 8 ± 1 | |
| Genes encoding proteins with diverse functions | ||||
| clpB | ClpB protein (heat shock protein) | 10.4 ± 0.4 | 9.4 ± 0.39 | |
| gst | Glutathione transferase | 7.4 ± 0.36 | 5.1 ± 1 | |
| hsdR | Type I restriction enzyme EcoKI R protein | 9 ± 1 | 9.7 ± 0.92 | |
| katG | Catalase HPI | 17 ± 0.06 | 12.3 ± 1 | |
| speG | Spermidine n1-acetyltransferase | 3 ± 0.25 | 5 ± 0.38 |