TABLE 1.
S. aureus plasmids and strains used in this study
| Plasmid or strain | Genotype and description | Reference |
|---|---|---|
| Plasmids | ||
| pRN5548 | Vector carrying a staphylococcal inducible PblaZ promoter | 14 |
| pRN6441 | Cloning vector | 14 |
| pRN6683 | agr P3-blaZ fusion | 4 |
| pRN6958 | agrD2 in pRN5548 | 3 |
| pGJ2001 | agrB1 in pRN5548ΔPstI | 18 |
| pGJ2002 | agrB2 in pRN5548ΔPstI | This study |
| pGJ4002 | agrD1 in pRN6441 | 18 |
| pLZ2004 | agrB1-6xhis in pRN5548 | 18 |
| pLZ2005 | agrB2-6xhis in pRN5548 | This study |
| pLZ2006 | Chimeric agrB (1P2) in pRN5548 | This study |
| pLZ2007 | Chimeric agrB (1H2P1) in pRN5548 | This study |
| pLZ2008 | Chimeric agrB (1H2) in pRN5548 | This study |
| pLZ2009 | Chimeric agrB (2P1) in pRN5548 | This study |
| pLZ2010 | Chimeric agrB (2H1P2) in pRN5548 | This study |
| pLZ2011 | Chimeric agrB (2H1) in pRN5548 | This study |
| pLZ2012 | Chimeric agrB (1C2) in pRN5548 | This study |
| pLZ2013 | Chimeric agrB (1D2) in pRN5548 | This study |
| pLZ2014 | Chimeric agrB (1E2) in pRN5548 | This study |
| pLZ2015 | Chimeric agrB (1F2) in pRN5548 | This study |
| pLZ2016 | Chimeric agrB (1G2) in pRN5548 | This study |
| pLZ2017 | Chimeric agrB (2C1) in pRN5548 | This study |
| pLZ2018 | Chimeric agrB (2F1) in pRN5548 | This study |
| pLZ2019 | Chimeric agrB (2C1F2) in pRN5548 | This study |
| pLZ2020 | Chimeric agrB (1C2F1) in pRN5548 | This study |
| pLZ2021 | Chimeric agrB (1Q2) in pRN5548 | This study |
| pLZ2022 | Chimeric agrB (2Q1) in pRN5548 | This study |
| pLZ2023 | Chimeric agrB (2H1P2Q1) in pRN5548 | This study |
| pLZ2024 | Chimeric agrB (2F1Q2) in pRN5548 | This study |
| pLZ2025 | Chimeric agrB (2F1H2) in pRN5548 | This study |
| pLZ2026 | Chimeric agrB (2F1Q2) in pRN5548 | This study |
| pLZ2027 | Chimeric agrB (2F1H2P1Q2) in pRN5548 | This study |
| pLZ2028 | Chimeric agrB (1F2H1) in pRN5548 | This study |
| pLZ2029 | Chimeric agrB (1P2Q1) in pRN5548 | This study |
| pLZ2030 | Chimeric agrB (1F2H1P2Q1) in pRN5548 | This study |
| pLZ4001 | agrD2 in pRN6441 | This study |
| Strains | ||
| RN6390B | Group I agr+ strain | 14 |
| SA502A | Group II agr+ strain, ATCC27217 | 3 |
| RN6911 | RN6390B agr 1057-4546::tetM | 14 |
| GJ2035 | RN6911(pI524) | 18 |
| GJ2001 | GJ2035(pGJ2001) | 18 |
| LZ4001 | GJ2035(pLZ4001) | This study |