TABLE 2.

Construction of chimeric AgrB expression plasmids^a

Method and plasmid	Chimeric agrB	Fragment 1		Fragment 2
		Template	Primersb	Template	Primersb
Enzymatic digestion-based subcloning
pLZ2006	1P2	pLZ2004-C		pLZ2005-D
pLZ2007	1H2P1	pLZ2008-C		pLZ2004-D
pLZ2008	1H2	pLZ2004-A		pLZ2005-B
pLZ2009	2P1	pLZ2005-C		pLZ2004-D
pLZ2010	2H1P2	pLZ2005-A		pLZ2006-B
pLZ2011	2H1	pLZ2005-A		pLZ2004-B
pLZ2024	2F1Q2	pLZ2018-C		pLZ2021-D
pLZ2025	2F1H2	pLZ2018-A		pLZ2005-B
pLZ2026	2F1Q2	pLZ2005-C		pLZ2021-D
pLZ2027	2F1H2P1Q2	pLZ2018-A		pLZ2026-B
pLZ2028	1F2H1	pLZ2015-A		pLZ2004-B
pLZ2029	1P2Q1	pLZ2004-C		pLZ2022-D
pLZ2030	1F2H1P2Q1	pLZ2015-A		pLZ2023-B
PCR-based subcloning
pLZ2012	1C2	pLZ2004	GJ#76/GJ#44	pLZ2005	GJ#78/GJ#45
pLZ2013	1D2	pLZ2004	GJ#75/GJ#44	pLZ2005	GJ#77/GJ#45
pLZ2014	1E2	pLZ2004	GJ#29/GJ#44	pLZ2005	GJ#49/GJ#45
pLZ2015	1F2	pLZ2004	LZ#4/GJ#44	pLZ2005	LZ#5/GJ#45
pLZ2016	1G2	pLZ2004	LZ#6/GJ#44	pLZ2005	LZ#7/GJ#45
pLZ2017	2C1	pLZ2005	LZ#36/GJ#44	pLZ2004	LZ#35/GJ#45
pLZ2018	2F1	pLZ2005	LZ#34/GJ#44	pLZ2004	LZ#33/GJ#45
pLZ2019	2C1F2	pLZ2005	LZ#36/GJ#44	pLZ2015	LZ#35/GJ#45
pLZ2020	1C2F1	pLZ2012	LZ#34/GJ#44	pLZ2004	LZ#33/GJ#45
pLZ1021	1Q2	pLZ2004	LZ#27/GJ#44	pLZ2005	LZ#26/GJ#45
pLZ2022	2Q1	pLZ2005	LZ#38/GJ#44	pLZ2004	LZ#39/GJ#45
pLZ2023	2H1P2Q1	pLZ2010	LZ#38/GJ#44	pLZ2004	LZ#39/GJ#45

^aThe chimeric AgrB expression plasmids were constructed by ligation of DNA fragment 1 and 2. A diagram of fragments A, B, C, and D is shown in Fig. 1.

^bThe oligonucleotide primers used are listed in Table 3.