TABLE 3.
Oligonucleotides used in this study
| Primer | Nucleotide sequence (5′→3′) | Location |
|---|---|---|
| GJ#29 | CGTAATTAACGTAAACAG | agrB1 (1944-1961)a |
| GJ#44 | CTATTATTCCATGGACTTCATTTAC | Around the NcoI site of pRN5548 |
| GJ#45 | GTAAATGAAGTCCATGGAATAATAG | Complementary to GJ#44 |
| GJ#49 | CACTTATCCTATATGTTAATACG | agrB2 (187-209)b |
| GJ#75 | AATATAGGCAATAGTATACAT | agrB1 (1911-1931)a |
| GJ#76 | TTTACCTATATTTTTAGCTAAG | agrB1 (1880-1901)a |
| GJ#78 | ATACTTGTAACATATAGTATTTC | agrB2 (127-149)b |
| LZ#4 | AAATGTTAAATTCGTAATTAAC | agrB1 (1952-1973)a |
| LZ#5 | ATGTTAATACGCTATAATG | agrB2 (199-223)b |
| LZ#6 | TCTTCTTATTAAATAAAATGTT | agrB1 (1869-1888)a |
| LZ#7 | AATGCACATGGTGCT | agrB2 (214-228)b |
| LZ#26 | AAGTATTTATCTATAATTATGTAT | agrB2 (424-447)b |
| LZ#27 | TTTTCGTTTAATAAGTCGCA | agrB1 (2179-2198)a |
| LZ#33 | TATTTAATAAGAAGACATGCAC | agrB1 (1974-1995)a |
| LZ#34 | ATAGGATAAGTGTGTAACT | agrB2 (180-198)b |
| LZ#35 | TTAATTGTTATGTATACTATTGCCT | agrB1 (1902-1926)a |
| LZ#36 | TTTAAAAAAATTACCAACTATAATTTGC | agrB2 (99-126)b |
| LZ#38 | TTTACGTTTTACTAATTTTATTGGT | agrB2 (399-423)b |
| LZ#39 | AAATATTATGCGATTATTGTTAGT | agrB1 (2199-2222)a |