. 2016 Aug 25;11(1):121–128. doi: 10.5009/gnl16010

Table 1.

Oligonucleotide Primers for Real-Time PCR

Gene	Primer	Primer Sequence (5′-3′)	PCR product (bp)
Albumin	Forward	GCACAGAATCCTTGGTGAACAG	100
Albumin	Reverse	ATGGAAGGTGAATGTTTCAGCA	100
ASGR1	Forward	CAGCAACTTCACAGCGACCA	96
ASGR1	Reverse	AGCTGGGACTCTAGCGACTT	96
AFP	Forward	AGACTGCTGCAGCCAAAGTGA	80
AFP	Reverse	GTGGGATCGATGCTGGAGTG	80
CK19	Forward	TCCGAACCAAGTTTGAGACG	186
CK19	Reverse	CCCTCAGCGTACTGATTTCC	186
Cyp1A2	Forward	CGGACAGCACTTCCCTGAGA	124
Cyp1A2	Reverse	AGGCAGGTAGCGAAGGATGG	124
TAT	Forward	TGGGGACCCTACTGTGTTTGG	105
TAT	Reverse	ATGGATGGGGCATAGCCATT	105
GAPDH	Forward	TGCACCACCAACTGCTTAGC	87
GAPDH	Reverse	GGCATGGACTGTGGTCATGAG	87

PCR, polymerase chain reaction; ASGR1, asialoglycoprotein receptor 1; AFP, alpha-fetoprotein; CK19, cytokeratin 19; Cyp1A2, cytochrome P450 superfamily of enzymes 1A2; TAT, tyrosine amino-transferase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.