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. 2004 Oct;186(20):6983–6998. doi: 10.1128/JB.186.20.6983-6998.2004

FIG. 2.

FIG. 2.

Detection of ParA-ParA, ParA-ParB, and ParB-ParB interactions with the yeast two-hybrid system (YTH). (A) Region of the genome used as the template in PCRs; genome coordinates define the primer sites. Coordinates shown correspond to P. aeruginosa genome coordinates. Primer A3 includes the ribosome-binding site for parA. Primers B3 and B4 introduce a BamHI site into the DNA sequence without changing the protein sequence. (B) Summary of results of the YTH assay. S. cerevisiae strain L40 was transformed with two compatible plasmids expressing only Gal4AD, LexA, or products of translational fusions between Gal4AD or LexA and ParA or ParB (parA was amplified with primers A1 and A2; parB was amplified with primers B1 and B2). −, no interactions (β-galactosidase activity of <0.2 U); +, weak interactions (β-galactosidase activity of 1 to 3 U); ++, strong interactions (β-galactosidase activity of >10 U).