FIG. 4.

Cross-linking of purified ParB and its truncated derivatives by glutaraldehyde. Proteins at a concentration of 0.1 mg/ml were incubated with various amounts of glutaraldehyde (GA). The samples were separated on homogeneous gels by SDS-12.5 or 20% PAGE and visualized by Coomassie blue staining (PHAST gel system). Lane O corresponds to the purified protein without GA; lanes 1 to 4 correspond to GA concentrations of 0.001, 0.002, 0.005, and 0.01% (wt/vol), respectively. m, monomer; d, dimer; h, higher-order complex. Lane M contains protein molecular weight markers; different molecular markers were used—one for the gels with ParB and C-ParB and another for the gels with N-ParB, N-ParB-C-KorB, and C-KorB.