TABLE 1.
Plasmids used in this study
| Category of derivatives | Plasmid | Relevant featuresa | Reference or source |
|---|---|---|---|
| pBBR1MCS1 | Cmrmob; BHR | 28 | |
| pJMB500 | pBBR1MCS1 derivative; lacIqtacp-parBPa | This work | |
| pGBT400 derivatives | pGBT400 | SmrlacIqtacp expression vector; IncQ rep | 20 |
| pABB100 | tacp-parB | This work | |
| pABB111 | tacp-parBΔ230-290 | This work | |
| pABB114 | tacp-parBΔ91-290 | This work | |
| pABB128 | tacp-parBΔ1-234 | This work | |
| pABB130 | tacp-parBΔ121-183 | This work | |
| pET28mod derivatives | pET28a(+) | Knr T7p expression vector; His6 tag T7tag pMB1 ori | Novagen |
| pET28mod | Knr T7p expression vector; His6 tag pMB1 ori | 21 | |
| pABB211 | T7p-parBΔ230-290 | This work | |
| pABB216 | T7p-parBΔ230-290-korBRK2Δ1-257 | This work | |
| pABB228 | T7p-parBΔ1-234 | This work | |
| pKLB28 | T7p-parB | This work | |
| pMLB6.0 | T7p-korBRK2Δ1-257 | 34 | |
| pGBT30 derivatives | pGBT30 | PnrlacIqtacp expression vector; pMB1 ori | 19 |
| pKLB1 | tacp-parA | This work | |
| pKLB2 | tacp-parB | This work | |
| pKLB3 | tacp-parAB | This work | |
| pABB311 | tacp-parBΔ230-290 | This work | |
| pABB316 | tacp-parBΔ230-290-korBRK2Δ1-257 | This work | |
| pABB323 | tacp-parBΔ1-18 | This work | |
| pABB324 | tacp-parBΔ1-53 | This work | |
| pABB326 | tacp-parBΔ1-142 | This work | |
| pABB328 | tacp-parBΔ1-234 | This work | |
| pABB330 | tacp-parBΔ121-183 | This work | |
| pGAD424 derivatives | pGAD424 | Pnrleu2 shuttle vector; pMB1 2μm ori gal4AD fusions | Clontech |
| pKLB1.4 | gal4AD-parA translational fusion | This work | |
| pKLB2.4 | gal4AD-parB translational fusion | This work | |
| pBTM116 derivatives | pBTM116 | Pnrtrp1 shuttle vector; pMB1 2μm ori lexADB fusions | Clontech |
| pKLB1.6 | lexADB-parA translational fusion | This work | |
| pKLB2.6 | lexADB-parB translational fusion | This work | |
| pABB511 | lexADB-parBΔ230-290 translational fusion | This work | |
| pABB512 | lexADB-parBΔ163-290 translational fusion | This work | |
| pABB513 | lexADB-parBΔ143-290 translational fusion | This work | |
| pABB514 | lexADB-parBΔ91-290 translational fusion | This work | |
| pABB515 | lexADB-parBΔ55-290 translational fusion | This work | |
| pABB526 | lexADB-parBΔ1-142 translational fusion | This work | |
| pABB527 | lexADB-parBΔ1-200 translational fusion | This work | |
| pABB528 | lexADB-parBΔ1-234 translational fusion | This work | |
| pABB529 | lexADB-parBΔ1-234-Δ284-290 translational fusion | This work | |
| pABB530 | lexADB-parBΔ122-184 translational fusion | This work | |
| pOG04 derivatives | pOG04 | Pnr pMB1/P7 rep; no par | 53 |
| pABB70 | tacp-parAB lacIq | This work | |
| pABB71 | parS2/3 | This work | |
| pABB72 | parS2/3tacp-parAB lacIq | This work | |
| pUC18 derivatives | pUC18 | Pnr pMB1 ori | 55 |
| pKLB181 | parS2/3 | This work | |
| pABB182 | parS1/4 | This work | |
| pABB183 | parS7II | This work | |
| pABB184 | parSSc | This work | |
| pGB2 derivatives | pGB2 | Smr Spr pSC101 rep par | 7 |
| pABB811 | parS2/3I | This work | |
| pABB812 | parS2/3II | This work | |
| pABB821 | parS1/4I | This work | |
| pABB822 | parS1/4II | This work | |
| pABB831 | parS7I | This work | |
| pABB832 | parS7II | This work | |
| pABB841 | parSScI | This work |
a
DB, DNA-binding domain; I, orientation I; II, orientation II.