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Effects of temperature on enzyme activity and stability. (a) Effects of temperature on stability. The enzyme solution was incubated in 10 mM potassium phosphate buffer, pH 7.0, at various temperatures, and the remaining activity was determined under standard assay conditions for decarboxylation. •, 50°C; ▪, 60°C; ▴, 70°C. (b) Arrhenius plot. The activation energy for the decarboxylation of γ-resorcylate to resorcinol was calculated from the Arrhenius plot.
