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Restriction fragment analysesa
| Restriction fragment (total size [kb]) | Fragment size (kb) |
|---|---|
| SpeI (1,390) | 280, 240, 150, 140, 125, 100, 80, 80, 75, 40, 30, 25, 15, 10 |
| PmeI (1,350) | 180, 150, 140, 130, 120, 110, 100, 90, 80, 75, 70, 50, 30, 25, 20, 15 |
Fragments sizes were estimated by comparison with either molecular weight standard or known S. meliloti SpeI and PmeI restriction fragments.
