FIG. 3.

Effects of frmR expression on levels of frmAB, measured with real-time PCR. Glucose overnight cultures of FBSC222 carrying the plasmids pACB/frmR, pACB/alt (denoted frmR + 7aa), or pACB/empty were inoculated into MOPS minimal medium with either arabinose (induced) or glucose (uninduced), and cDNA was isolated. SYBR Green real-time PCR was performed, and data from each sample were normalized to the housekeeping gene frr. The quantity of each transcript is expressed relative to the negative control (the empty vector strain amplified with frmR primers). Each panel shows the results from one primer pair. Error bars representing the standard deviations of three replicates were too small to be visible for some samples.