FIG. 4.
Induction of frmRAB with formaldehyde, as measured with real-time PCR. WT strain MG1655 was grown to an OD600 of 0.15 in 0.01% glucose plus 0.1% arabinose and then split into three cultures containing no inducer, 0.25 mM formaldehyde, or 0.5 mM SNOG, and cells were harvested to make cDNA 30 min later. Relative real-time PCR and normalization were performed as for Fig. 3. The quantity is given relative to the negative control from Fig. 3, and so the data from both figures are comparable. Standard curves were generated from the same DNA.