TABLE 3.

Phenotypic analysis

Substrate	Plate	Testa	Well	Log2 ratio	WT ODb	FB23401 OD
Tween 80	PM5	Supplement	H12	−4.8	1.0 ± 0.5	0.0
2′-Deoxycytidine	PM5	Supplement	D12	−4.7	1.2 ± 0.2	0.0
α-Methyl-d-glucoside	PM2	C source	C6	−3.6	1.0 ± 0.7	0.1
2′-Deoxylnosine	PM5	Supplement	C12	−3.6	1.1 ± 0.4	0.1
d-Lysine	PM3	N source	C7	−3.0	0.9 ± 0.4	0.1
m-Inositol	PM5	Supplement	G12	−2.6	1.0 ± 0.3	0.2
Thiophosphate	PM4	P source	B1	−2.4	1.5 ± 0.9	0.3
N-Phthaloyl-l-glutamate	PM3	N source	D2	−2.2	0.5 ± 0.1	0.1
Agmatine	PM3	N source	D12	−2.2	0.7 ± 0.6	0.1
α-Keto valeric acid	PM2	C source	E10	−2.0	0.5 ± 0.3	0.1

^aDifferent BIOLOG plates were designed to test for carbon, nitrogen, or phosphorous sources or the effects of a growth supplement.

^bCells were inoculated into BIOLOG plates as per the manufacturer's instructions, grown overnight at 37°C, and then the OD₅₉₅ was measured in a plate reader. Data are shown from five replicates of WT (± SD) and one of FB23401 (disrupted at frmB).